I am trying to Isolate small RNA from cotton anther, I used trizol method and mirvana kit but I didn't get the result, now I am moving to Qiagene kit, has anyone already used this Kit?
Qiagene Kit is very good. Try to keep everything according the producer's protocol. The quick start protoicol is provided with the kit. BTW which kit you are going to use?
No, I worked with it long ago and my impressions were very positive. If you have well fixed samples you do not need to worry. Anyway, be careful with the amount of tissue. Sometimes, if you add too much RNA is poorly cleaned.