Hi, can anyone help me to find the best HPLC method for quantification of desferrioxamine in urin and/or plasma?????

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Bruce Neagle

Did you perform an internet (Google) search 1st? Because of the low levels it probably an MS (as in LC-MS) detector! What was the method cited in the clinical studies or the 'product information sheet'?

Narong Chamkasem

What did you get when you search on the internet? That would be my starting point?.

Oliver Scherf-Clavel

Hi,

since the compound does not contain a good chromophore, I suggest you search for methods using "derivatization" unless you have a QqQ at hand (then search for LC-MS/MS methods.

regards

Oliver

I would chelate the sample with iron then run a LC-MS spectrum (before and after) using iron as my target.

I found this on the internet, but it looks like there is a confusion between Feroxamine and Deferoxamine structures.