Hi can anybody please tell me how can we elute the intact protein from SDS-Page gel...?? is there any standard protocol available ??

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Satish Pandey

Yes Sigma provide elution kit for protein isolation from the PAGE..

Hasan Mohammad

https://www.researchgate.net/post/What_is_the_best_way_to_extract_whole_proteins_from_SDS-page_for_mass_spect_analysis

may be you want to look at this thread, if your application is similar

Dominique Liger

Hi there,

This paper should be helpful:

http://www.ncbi.nlm.nih.gov/pubmed/22585504