I try to mark Proximal tubular cells with LTL while doing immunofluorescence staining.
I use BioWorld LTL-Texas Red. I tried 1:100 for 1 h after secondary antibody and 1:50 overnight with primary antibody. Both conditions didn't give good staining. any suggestions?
Did you ever find a solution for this?
I purchased LTL-Cy5 from bioworld and do not see any staining in my kidneys… i have tried several different concentrations, incubation times (RT and 4C) and nothing has worked.
Hi Joshua Giblin . I had to move to another company, which has many citations and changed the colours of my secondary antibodies accordingly.
Lectins: Lotus Tetragonolobus Lectin (LTL), Fluorescein labe (vectorlabs.com)
I used it 1:100 with 2ry antibodies. Incubated 1h at r.t.
It worked very nice! Good Luck!
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