I've searched for protocols to derive BMMs and most say that you use L929 conditioned media to obtain BMMs (example paper - weischenfeldt and porse (2008) csh protocols). The L929 is a source of m-csf to mature macrophages. However, Verreck et al., (2004) (PMID - 15070757) say that m-csf matures macrophages to an m2 phenotype (this work on humans). SO, just wanting to know if the l929 matured BMMs will be of an m2 phenotype only?

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