4 Questions 16 Answers 0 Followers
Questions related from Shaumick Bhattacharjee
I am trying to collect peritoneal macrophages for experiments. I notice that when I take a lavage 6 days post thioglycollate injection, i.p and FACS phenotype them with F4/80 and Ly6G, I get...
30 March 2013 9,704 23 View
I'm trying to identify the effect of different cytokines on the level of F4/80 expression in macrophages from the peritoneum that may be produced when sterile inflammation is induced. I understand...
29 January 2013 1,818 4 View
I've searched for protocols to derive BMMs and most say that you use L929 conditioned media to obtain BMMs (example paper - weischenfeldt and porse (2008) csh protocols). The L929 is a source of...
30 December 2012 1,687 5 View
I culture my human monocyte derived macrophages in Nunc Upcell® plastic plates and lift them in 5mM EDTA/PBS at 4 degrees, and am able to lift most of the cells successfully. However, because of...
29 November 2012 364 8 View