Shaumick Bhattacharjee

4 Questions 16 Answers 0 Followers

Questions related from Shaumick Bhattacharjee

Troubleshooting help on getting murine peritoneal macrophages

I am trying to collect peritoneal macrophages for experiments. I notice that when I take a lavage 6 days post thioglycollate injection, i.p and FACS phenotype them with F4/80 and Ly6G, I get...

30 March 2013 9,707 23 View

Does anyone know what cytokines produce during sterile inflammation in a model of peritonitis induced by 3% thioglycollate?

I'm trying to identify the effect of different cytokines on the level of F4/80 expression in macrophages from the peritoneum that may be produced when sterile inflammation is induced. I understand...

29 January 2013 1,821 4 View

Hi all, might be a stupid question, but wanted to know if bone marrow derived macrophages (BMM) can be made to display an m1 or m2 phenotype? Thanks!

I've searched for protocols to derive BMMs and most say that you use L929 conditioned media to obtain BMMs (example paper - weischenfeldt and porse (2008) csh protocols). The L929 is a source of...

30 December 2012 1,692 5 View

How can you prevent macrophages from clumping, once lifted from culture?

I culture my human monocyte derived macrophages in Nunc Upcell® plastic plates and lift them in 5mM EDTA/PBS at 4 degrees, and am able to lift most of the cells successfully. However, because of...

29 November 2012 367 8 View