Hello, what should the concentration of NEM (N-ethylmaleimide) be to block thiol groups on PBMCs?

03 March 2014 1 3K Report

Thiol blockers.

Article Differential inhibition of human T-lymphocyte activation by ...

We use a final concentration of 20 mM (from a 0.5M stock prepared in ethanol) in our experiments with bacterial proteins. Hope that helps!

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