Do you think that proinflammatory cytokines such as TNF-alpha, IL-1beta and IL-6 may act differently in the prefrontal cortex and hippocampus?

Hello; In terms of learning and memory, do you think that proinflammatory cytokines such as TNF-alpha, IL-1beta, and IL-6 may act differently in the prefrontal cortex and hippocampus? I've some...

08 September 2020 7,032 3 View

What is the best way for analysing the acquisition trials in Morris Water Maze?

Hello! I performed Morris Water Maze with 5 days acquisition and a sixth-day probe test in rats. Rats had 4 trials per day. Data analysis is confusing. Most of the protocols said that...

11 April 2020 7,484 10 View

What are the growing topics in algebraic combinatorics and graph theory for research?

I need some suggestions what are the growing topics in algebraic combinatorics and graph theory for research? Thank you in advance to everyone who will answer.

21 November 2019 9,552 6 View

How can we use ECDA(Eicosadienoic acid) to detect amines?

Hello! My coworker have been working with spoilage tests and Ive been helping him. But these days we have a problem about ECDA solution. We couldnt get any colour change when we test it. So I hope...

06 November 2019 9,543 2 View

Does Kapa genotyping kit suitable for TA Cloning ?

Hello, I am extracting mouse DNAs and performing PCRs through KAPA 2G Fast hotstart genotyping kit. Before I use the pcr products for TA Cloning (invitrogen), I am purifying PCR products through...

14 October 2019 2,984 2 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View