Do you think that proinflammatory cytokines such as TNF-alpha, IL-1beta and IL-6 may act differently in the prefrontal cortex and hippocampus?

Hello; In terms of learning and memory, do you think that proinflammatory cytokines such as TNF-alpha, IL-1beta, and IL-6 may act differently in the prefrontal cortex and hippocampus? I've some...

08 September 2020 7,032 3 View

What is the best way for analysing the acquisition trials in Morris Water Maze?

Hello! I performed Morris Water Maze with 5 days acquisition and a sixth-day probe test in rats. Rats had 4 trials per day. Data analysis is confusing. Most of the protocols said that...

11 April 2020 7,484 10 View

What are the growing topics in algebraic combinatorics and graph theory for research?

I need some suggestions what are the growing topics in algebraic combinatorics and graph theory for research? Thank you in advance to everyone who will answer.

21 November 2019 9,552 6 View

How can we use ECDA(Eicosadienoic acid) to detect amines?

Hello! My coworker have been working with spoilage tests and Ive been helping him. But these days we have a problem about ECDA solution. We couldnt get any colour change when we test it. So I hope...

06 November 2019 9,543 2 View

Does Kapa genotyping kit suitable for TA Cloning ?

Hello, I am extracting mouse DNAs and performing PCRs through KAPA 2G Fast hotstart genotyping kit. Before I use the pcr products for TA Cloning (invitrogen), I am purifying PCR products through...

14 October 2019 2,984 2 View

Hello, Is there anyone used erythropoietin from Prospect firm with CYT 325 code?

I want to use it at cell culture for induction. Can you help me ?

19 February 2018 6,023 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

For gelatin coating for culturing HUVEC.Should the gelatin be made in sterile water or PBS? Do I need to autoclave the gelatin soln after making it ?

I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?

28 February 2021 274 2 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

How to dissolve cross-linked Amberlite IRC120 H, hydrogen form resin?

Hello all, I have used Amberlite IRC120 H, hydrogen form acidic cation-exchange resin for the ion-exchange of waterglass [sodium silicate solution -- 40% Sodium silicate (mol ratio > 3.2)...

21 February 2021 5,614 3 View

Best protocol for immunogold using araldite resin ?

Hello, We perfom Tokuyasu method (ultrathin cryo sectionning) very often to make immunogold experiments. This time, we need to make a PLT technique with K4M or LR white resin. Our results were...

14 February 2021 4,959 1 View

How to improve the adhesion of epoxy coating on steel substrate? Which factor affects?

I am facing a problem regarding a de-adhesion problem with the coating. A brief description of my methodology is given below. I am using mild or Low Carbon steel as a substrate and I polish the...

14 February 2021 2,167 4 View

Does any one have experience with Autoclavable cell culture media (RPMI 1640/DMEM)? Does it work well?

12 February 2021 2,958 1 View

Purified protein requires Calcium for activation, but the elution buffer I used seems to interact with Calcium, help?

09 February 2021 6,946 4 View

What recipe of homemade lenti-virus concentrator do you use?

My lab is using a lenti-X concentrator. And we are looking for a homemade recipe. I have tried this recipe....

08 February 2021 5,965 1 View