I am looking for some method for phage inactivation with out damage of bacteria, for sterility test of phage preparation by USP 71.
Thanks in advance
Usually people want to do the opposite and inactivate the bacteria without damaging the phage in order to make. sterile phage solution. I think there will not be a simple answer to your question because different phages will have very different sensitivities to inactivation so it will depend upon the phage.
Can you elaborate? are you trying to block a specific phage or phages in general (much harder)? what level of inactivation are you looking for? Elene Lomadze
Mohammad Al Madadha For manfacturing phage preparate it is nessesary to perform sterility test of preparate. So to perform it is nessesary to inactivate some antimicrobal properties of preparation, because in case of contamination, preparate antimicrobal properties may have some impact on its detection.
Preparete is coctail of several bacteriophages of different bacteria.
Michael J. Benedik yes i know, Our preparate is coctail of several phages of different bacteria. Due to GMP standards when streility test is performed, antimicrobal properties of preparate must have been inactivated for detection any contamination. so I am looking for any method to unactivate phages with out damage of bacteria.
I think I better understand. You have your phage cocktail and wish to inactivate it really as a negative control but using a reagent that will not later be toxic to bacteria?
I think the best approach is irradiation, either UV or gamma irradiation or even X-ray, depending upon what you have access to. Heat would work with some phage but not necessarily with all. Some small phages survive at 95c although many are inactivated at 65C
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