Hello, I'd like to 35S (Cys/Met) radio-label a protein being expressed in P. pastoris.Does anyone have a recomended protocol.
Thanks
Have you tried a yeast-like protocol?
On what basis what their views approved, how were they able to influence the world civilization of today and the example of people I'm talking about are HERODOTUS AND LEOPOLD VON RANKE AND MANY MORE.
26 April 2023 4,244 2 View
I need questionnaires for the research topic EFFECT OF INFORMATION TECHNOLOGY ON THE EFFICIENCY OF TAX ADMINISTRATION IN NIGERIA: A CASE STUDY OF FEDERAL INLAND REVENUE SERVICE.
14 February 2023 7,826 0 View
Hi, I am trying to detect Parkin (a mitochondria related protein) on western blot however my antibody doesn't detect it at the right location (50KDa) but detects a double band at 110KDa. I used...
10 October 2022 4,944 2 View
I am trying to model the effects of trade liberalization/ openness, income inequality on poverty reduction.
25 May 2022 2,412 8 View
Many of the adolescents attending the STI clinics and youth friendly centres opt for the HIVST but they usually fail to disclose their results. Can you share your experience of our optimal...
17 January 2021 8,465 2 View
I want to separate a complex mixture of esters into medium and high molecular weight esters using solvent extraction.
11 October 2020 10,173 2 View
Can anyone recommend good tutorial materials and literature on 'grid forming and grid following converter models'?. I will appreciate your recommendations and suggestions. Thanks.
02 July 2020 9,544 3 View
I will like to model the microstructure of concrete/mortar, is there any software to model the resulting products? Thanks
20 January 2019 1,265 2 View
PVA fibers are modified with oiling agents to reduce bonding between the fiber and the cementitious matrix. What is the best type of oiling agent and method of application is most...
05 December 2018 2,199 1 View
I'm using design expert software for the design of the influence of different factors in my experiment. However, I couldn't get any section that shows the percentage contribution of each factor on...
29 November 2018 6,280 3 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...
27 July 2024 6,605 0 View