Hello! Based on the concentration you've provided, it's possible to use both liquid and powder forms of bovine insulin to make your cell culture medium.

If you choose to use the liquid form of bovine insulin, you will need to dilute it to the appropriate concentration using a suitable solvent, such as water or a buffer. It's important to follow the manufacturer's instructions for dilution, storage, and handling of the liquid insulin to ensure its stability and effectiveness.

If you choose to use the powder form of bovine insulin, you will need to reconstitute it in a suitable solvent to make a stock solution, which can then be further diluted to the desired concentration. Again, it's important to follow the manufacturer's instructions for reconstitution and storage to ensure the stability and effectiveness of the insulin.

In either case, it's important to sterilize the insulin solution before adding it to the cell culture medium. This can be done by passing the solution through a sterile filter or autoclaving it, depending on the method that is most appropriate for your particular situation.

Regarding the other components of your cell culture medium, it's important to ensure that they are also prepared and added in a sterile manner to avoid contamination of the cells. The final concentration of each component should be based on the specific needs of the cells you are culturing and the experimental conditions you are using.

I hope this helps, and please let me know if you have any further questions!