Hello! Has anyone ever transfected HCC1569?

More Nuria Arroyo-Garrapucho's questions See All

¿Cómo se aborda metodológicamente desde el trabajo social el estigma y la discriminación asociados a las drogodependencias?

Con esta pregunta se pretende identificar los métodos y técnicas más adecuadas para abordar las razones por las que las personas drogopendientes totalmente rehabilitadas, se encuentran en...

10 March 2024 6,561 2 View

Why do I get so many truncated versions of my recombinant protein?

Hi everyone! I am expressing two 250kDA fusion proteins in E.coli BL21. They have been codon optimized (once) for E.coli expression but it seems that we can't purify only one band. Even after...

27 March 2023 8,476 5 View

How to set up a PBC to encapsulate a nanoparticle in Materials Studio?

Hello everyone! I have built a gold nanoparticle and I want to perform a DFT calculation (geometry optimization) of this nanoparticle in Materials Studio (Dmol3). But, in order to calculate the...

22 August 2022 9,389 0 View

Dear All, Where can I download daily photosynthetically active radiation (PAR) total data for free?

I need from 2022, March to actually. I was using the https://power.larc.nasa.gov/data-access-viewer/, but as of March 2022 no data has been recorded. Thanks !!

06 July 2022 1,267 0 View

CRISPR/Cas9 trouble with SantaCruz's: CRISPR/Cas9 KO plasmid?

Hi everyone! I am trying to knock-out a gene in breast cancer cell lines with CRISPR/Cas9 technology. I have used commercial CRISPR/Cas9 system from SantaCruz. SantaCruz's: CRISPR/Cas9 KO...

16 November 2021 9,836 3 View

Sources of self-efficacy questionnaire?

Greetings, I have been desperately searching for a questionnaire / survey for Bandura's four (4) sources of self-efficacy questionnaire applicable to graduate students (or more drilled down,...

23 March 2021 7,449 0 View

Someone knows how to preconcentrate bacteria density in water samples?

I have low bacteria density in water samples. I thought to preconcentrate them by filtrating a known volume of water (e.g. 500 ml) in 0.2 um Nylon filters, and then suspended bacteria that has...

14 January 2021 1,057 2 View

How to conduct Pearson R using SPSS?

How to conduct a Pearson R with an instrument that has 18 questions (five predictor variables0 and another instrument that measures job satisfaction. Research Questions R1: Is there a...

10 April 2020 408 2 View

Why in standard curve qPCR the most concentrate cDNA sample doesn't work and serial dilutions are correct?

Hi! I did a standard curve of my gene of interest. For this, I started with 100ng of cDNA and did serial dilutions (1:10) of this sample 3 steps. The Ct of this samples are: 100ng --> 32,62...

05 November 2019 3,281 3 View

Problems with transfecting fibroblasts?

Hello I am writing because I am having serious problems transfecting foreskin human fibroblasts with several plasmids at a time. At first we started using the transfection reagent Trans-IT-2020...

01 April 2019 1,439 4 View

AMAXA Lonza nucleofection lower volume of buffer?

Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?

07 August 2024 4,616 0 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Issues with Protein Expression in Transfected cells?

Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...

30 July 2024 6,648 1 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

Does anyone have experience electroporating SKOV3 cells with a large plasmid using Neon transfection?

I have been trying to electroporate SKOV3 cells with a large plasmid (11kb) without much success. Any tips?

29 July 2024 3,229 1 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

Can anyone help me explain my problem?

Hi, Now I am doing a single-base editing by using HiFi Cas 9, guideRNA, single stranded DNA, HDR Enhancer V2 and my tranfection mehtod is lipofection (RNAiMax). Unfortunately, after gene editing,...

26 July 2024 2,136 0 View

Can I use Polyjet after its expiration date?

I have a Polyjet that has passed its labeled expiration date for 1 year and I'm going to re-start transfection experiments. It is really needed to change it? Transfection efficiency may be lower?

23 July 2024 8,059 1 View

How to transfect circular mRNA to T cells?

Hello! We are using LNP to deliver circular mRNA into T cells. However, compared with linear mRNA, the transfection efficiency and protein expression duration are similar. Are LNP generation or...

21 July 2024 4,322 1 View