Detection of mouse T cell proliferation in PBMC culture
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,726 6 View
In reference to antibiotic as a commodity, what is your take and way forward on common molecules in both Veterinary and Human antibiotics in relation to currently experienced emergency of AMR?
20 July 2024 4,020 0 View
Dunn's equation should not be used in the case of a battery-type electrode, according to a paper published in the journal "Phys. Chem. Lett. 2020, 11, 9846–9849 (ref. attached)" that I recently...
12 May 2024 3,261 0 View
Hello everyone,I am currently working with the multivariate time series forecasting by using NeuralProphet to predict the price of product.I used 7 economic indicators as regressor in my...
22 March 2024 8,264 3 View
I would like to know that prophet time series model is under the category of neural network or machine learning or deep learning? I want to forecast the price of product depending on other...
19 February 2024 1,243 1 View
Dear RG users, I hope all of you are doing well. I just wanted to know how can I do dispacement controlled cyclic triaxial tests using ELDYN dynamic equipment. As you can see in the figure, there...
14 September 2023 2,217 0 View
generally polar basis set will provide the tensors (dipole moment,polarizability and hyperpolarizability). but how to find their contributions separately
24 June 2023 1,295 6 View
To study the effects of cell conditioned medium (CM) in animals in vivo, is it necessary to concentrate the medium after collection? I am not familiar with the preparation of the CM.
01 March 2023 695 3 View
These two models take a lot of time to train with powerful GPUs and required a minimum 512 batch size for good model performance.
28 February 2023 1,654 1 View
Width of depletion region which can be remarked using width of space charge layer (WSCL).
06 February 2023 2,621 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,822 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,104 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 639 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,891 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
