Adamska Mr

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Questions related from Adamska Mr

How to determine the positive cell population in flow cytometry using indirect staining?

I‘ve been using an unconjugated mouse anit rat anitbody to stain stem cells. The antibodies are scarce so unconjugated format is the onyl one we could obtain. we used a secondary antibody FITC....

07 September 2024 9,017 1 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

07 August 2024 7,711 6 View

Why is cell conditioned medium concentrated after collection? For transplantation experiments, can it be used without concentrating it?

To study the effects of cell conditioned medium (CM) in animals in vivo, is it necessary to concentrate the medium after collection? I am not familiar with the preparation of the CM.

02 March 2023 895 3 View

About What pore size cell strainer should I use to obtain a single cell suspension ?

Any suggestions will be much appreciated.

21 June 2021 1,169 3 View

Hello everyone. In QPCR, what would be some relevant murine genes (pan T cell genes) to detect proliferation of murine T cells in a PBMC culture?

Detection of mouse T cell proliferation in PBMC culture

10 April 2019 7,091 0 View