To study the effects of cell conditioned medium (CM) in animals in vivo, is it necessary to concentrate the medium after collection? I am not familiar with the preparation of the CM.
Hello Adamska Mr
Conditioned medium (CM) contains various paracrine factors secreted by cultured cells such as growth factors and cytokines. However, for CM to be effective in animals (in vivo) for the desired purpose, it is necessary to increase the concentration of the specific growth factors/cytokines that are present in it. This is possible only by concentrating the CM. If you do not concentrate the CM, an insufficient amount of molecules (growth factors/cytokines) in the CM may lead to poor outcome.
However, in the process of concentrating the CM in order to increase the concentration of growth factors, there is a possibility that it may alter the proportion of various molecules, and may not specifically increase the concentration of the factor that is crucial for the experiment.
Best.
Cell conditioned medium is concentrated after collection to increase the concentration of growth factors, cytokines, and other bioactive molecules secreted by cells. These molecules are typically present in low concentrations in the conditioned medium, and concentrating the medium can make them more effective in promoting cell growth, differentiation, and tissue regeneration.
Concentrating the conditioned medium can be achieved using different methods, such as ultrafiltration or centrifugation, which remove excess solvent and increase the concentration of the desired molecules.
For transplantation experiments, it is possible to use cell conditioned medium without concentrating it, but the efficacy of the treatment may be reduced. The concentration of bioactive molecules in the medium may not be high enough to achieve the desired therapeutic effect, especially if the target tissue is large or has a high cell density. Concentrating the medium can improve its potency and increase the chances of successful transplantation.
In summary, concentrating cell conditioned medium is important to increase the concentration of growth factors and other bioactive molecules, making it more effective in promoting cell growth, differentiation, and tissue regeneration. While it is possible to use the medium without concentration, the therapeutic effect may be reduced, especially for transplantation experiments targeting larger or denser tissues.
@Malcolm Nobre and Ahmad Al Khraisat, Thank you very much for your kind and thorough explanations. I understood the necessity of concentrating the medium. I will definitely consider this in my experimental plan.
The conditioned medium will be used to study bone regeneration.
Much appreciated.
Best wishes.
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