Hello everyone! I have a little task. Can you help me knock in a gene carrying a single nucleotide mutation using CRISPR?

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Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Do you know some program or app that allows me to build graphics in macbook air?

Hi everyone. Can somebody help me? I am looking for some program or app that allows me to build graphics of DRX, Raman, EIS, IR data, among others in my macbook air. When I had my windows PC it...

23 March 2019 3,216 2 View

Detection of Myc-tagged protein by anti-Myc but not by the specific antibody?

Hello everyone, I am using lentiviruses to overexpress proteins (Myc-tagged versions) of interest in hepatoma cells. After several rounds of selection with G418, all the untransduced cells were...

03 October 2017 3,331 5 View

Hello, sciencie people, So, I have het mouse carrying a point mutation. I PCR amplified the DNA and sanger sequenced. Will the mutation show?

The mutation is a G to an A.

19 July 2016 4,922 9 View

EMSA assay at pH4??

I have to do an EMSA assay with a protein of pI 6. Is it possible to carry out the assay at pH of 4, or such an acid buffer may alter DNA-DNA or DNA-protein interactions? Or may be could be better...

23 June 2013 3,496 1 View

Does anyone know of a good application to read DjVu files on macbook?

Hello, I'm looking for some versions of free software for macbook, among those I need an app that allows me to read files in DjVu format. Can you recommend any? I also need access to Comsol,...

01 January 1970 7,341 2 View

Dear Andrey: Have you read David w. Anthony's book? If you have, what is your opinion?

I read the book and I found it very interesting. Best, Julio

01 January 1970 962 5 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View