Hello, and first of all I hope you are well. I am trying to extract DNA from the bacteria linked to the marine copepod Parvocalanus crassirostris, which is a small crustacean about 400 microns total lenght. The amount of organisms that I have per sample is around 200, with an approximate weight of 1 mg of total biomass per sample. The kit I am using is the Zymobiomics DNA miniprep kit, but the results so far are not good despite some variations in the extraction protocol. Any tips regarding copepod DNA extraction or optimization of the Zymobiomics DNA miniprep kit with low biomass would be appreciated.
Greetings