Ariadna Amador

2 Questions 4 Answers 0 Followers

Questions related from Ariadna Amador

Hello everyone! I have a little task. Can you help me knock in a gene carrying a single nucleotide mutation using CRISPR?

I need to locate the mutation in the gene. I know the amino acid, the position number and the aminoacid I need to change it to. But how do I locate it in the gene? Thanks, Ariadna

01 September 2016 5,271 2 View

Hello, sciencie people, So, I have het mouse carrying a point mutation. I PCR amplified the DNA and sanger sequenced. Will the mutation show?

The mutation is a G to an A.

20 July 2016 5,081 9 View