The deep freezer (-80 degree) which we use to cryopreserve our mammalian cell culture stocks has confronted a problem, owing to which we are planning to shift the stored cell stocks to -20 degree. I don't have the experience where I had revived the cell stocks stored at -20. Any one among you having any experience about storing your cryo stocks at -20 degree without affecting cell viability?
Your answer will be of high importance
Thanking you in advance
Hi,
Probably if you store the cells for a long time they will loose viability. I will think that overnight is the longest that you should store them at -20. Could you store them in liquid nitrogen ?
Hi Dr Rivera,
Dear Sir thanks a lot for sharing your view. Liquid nitrogen storage is the best option than storing even at -80. But the problem is that we don't have a liquid nitrogen tank to transfer the cells to it.
How ever, we managed to shift the cells to other -80 degree unit.
Anyways, thanking you once again
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