Difference between the Coupled channel code CCFULL and FRESCO; which is better?
CiteScore calculation is based on Scopus data, while Impact Factor is based on Web of Science data. For example, Journal of Intellectual Capital has a CiteScore of 8.6 and an Impact Factor of...
01 July 2020 5,536 6 View
Hi everyone I isolated some plasmids, using alkaline lysis method (which I have been following for 2-3 years now) and the final plasmid pellet after washing in 70% ethanol was dried. Then I added...
23 December 2018 6,825 2 View
Hello.... I am intending to mimic hypoxia induced conditions in cancer cells with CoCl2 (for 24 hrs) and then re-oxygenate the CoCl2 treated cells with fresh culture media without CoCl2. I am...
03 April 2017 7,831 1 View
I am intending to stabilise HIF 1 aplha expression in cancer cells; I mean to say to induce hypoxia mimetic environment. Since we don't have a hypoxic chamber availability, we are intending to go...
30 March 2017 1,443 5 View
Hi I have to execuite some protein expression analysis under hypoxic conditions in cancer cells. Can anybody suggest me the most reliable way to induce and mimic hypoxic conditions in cell...
31 October 2016 4,529 3 View
I just need an assistance about how to develop a blot , using luminata forte and Xray cassete? Like the conditions in which the developer, fixer or the luminata forte to be opened... Is complete...
22 October 2016 3,534 3 View
I am looking to measure the co-factor NADPH produced by the cells, especially in the neutrophils after my treatment. Is there any particular colorimetric or fluorimetric assays are there to...
22 May 2016 7,928 1 View
I am searching for protocol for this experiment to conduct practical, in some experiment it acts as activator to enzyme amylase, i just want to assess how far it is going to affect the activity of...
22 September 2014 8,466 1 View
Hi all I have been facing a lot of problems with my western blot development. 1) First I failed to develop my target protein on x-ray although every time I got a fine band for my control(beta...
01 January 1970 4,937 1 View
To the cell culture handling folks, If I ask you, Which flask is better for your cell culture? or What parameters or specifications you look in a flask? what will be your answer. For example,...
01 January 1970 7,103 6 View
How to do Piezoelectric coupling in ANSYS-APDL or COMSOL between tapered substrate and rectangular piezoelectric patch? Piezoelectric coupling between the rectangular substrate and rectangular...
02 March 2021 9,833 3 View
I'm working on organotypic cerebellar slice cultures from postnatal rats and I'm not sure how to tell if the slices are healthy at any given point. I know there should be a lot of microglial...
15 February 2021 2,830 2 View
I need to couple Dopamine by NH2 end to silica nanoparticles coated by PEG-NH2, but first i need to convert the PEG-NH2 to COOH by using succinic anhydrous ... can you suggest a reaction condition ?
13 February 2021 2,768 4 View
hello i am trying to study the mechanical deformation due to the humidity on a 3d volume formed of 2 phases. my equation are dw/dt =grad(Dm*div(w)) and the mecanical is sigma=E*beta*nabla(w)....
27 January 2021 5,201 1 View
I have two discrete rigid bodies. A axial connector was established to connect two reference points. Now the task is to attach the reference points to the respective rigid part. How should I do...
25 January 2021 5,079 3 View
Currently I am reading one research paper regarding silicon based two and four mode division multiplexing for optical data transmission in 2 micrometer waveband. While reading I stuck at one...
21 January 2021 5,864 4 View
I am trying to model sloshing water in a tank in ANSYS Fluent using Wall motion. BUT it seems that System coupling is not responding to wall motion and it works only with Dynamic mesh.
20 January 2021 2,963 3 View
Are there analog circuit designers? Here is a small task for you. The active circuit below aims to improve the AC coupling in the biosignal amplifiers. Is that so? Are there any pros? I'm...
17 January 2021 3,548 14 View
What's the difference between light absorption and coupling in plasmonic metals? It seems that light absorbed in metal nanostructure/nanoparticles doesn't contribute to enhancement in electric...
14 January 2021 1,884 4 View
Hello, plese give me your idea! I simulate one slab concrete affected by temperature at top and bottom surfaces. after that, have a static load distribution with small area put on top surface. I...
12 January 2021 5,769 4 View