Rayees Padder

8 Questions 10 Answers 0 Followers

Questions related from Rayees Padder

Reason why my Plasmids isolated using alkaline lysis didn't dissolve in water?

Hi everyone I isolated some plasmids, using alkaline lysis method (which I have been following for 2-3 years now) and the final plasmid pellet after washing in 70% ethanol was dried. Then I added...

24 December 2018 6,925 2 View

Hello , While doing fluorescence microscopy to visualise cell organelle, is it mandatory to use antifade mountant (Prolong gold) or DPX can work too?

I am doing mitochondrial imaging analysis in adherent cells, using a fluorescent dye- Mitotracker Red CMXRos. Is it necessary to use Prolong Gold antifade mountant or it is fine to mount by DPX...

20 April 2017 1,732 4 View

What is the best cell confluence at which we should treat our running cancer cells with CoCl2 to mimic hypoxia mediated conditions?

Hello.... I am intending to mimic hypoxia induced conditions in cancer cells with CoCl2 (for 24 hrs) and then re-oxygenate the CoCl2 treated cells with fresh culture media without CoCl2. I am...

04 April 2017 7,916 1 View

Hello everybody... What is the shelf life of CoCl2 solution made to induce HIF1 stability in cancer cells?

I am intending to stabilise HIF 1 aplha expression in cancer cells; I mean to say to induce hypoxia mimetic environment. Since we don't have a hypoxic chamber availability, we are intending to go...

31 March 2017 1,535 5 View

Hello every body, Can the mammalian cell stocks be stored at -20 degree Celsius and if so how long will be the cell stocks viable at this temperature?

The deep freezer (-80 degree) which we use to cryopreserve our mammalian cell culture stocks has confronted a problem, owing to which we are planning to shift the stored cell stocks to -20 degree....

03 March 2017 1,365 3 View

Most reliable way to induce hypoxic condition in cancer cells?

Hi I have to execuite some protein expression analysis under hypoxic conditions in cancer cells. Can anybody suggest me the most reliable way to induce and mimic hypoxic conditions in cell...

01 November 2016 4,592 3 View

Hello, can anyone assist me about the exact conditions to develop a western blot, using Luminata forte?

I just need an assistance about how to develop a blot , using luminata forte and Xray cassete? Like the conditions in which the developer, fixer or the luminata forte to be opened... Is complete...

23 October 2016 3,625 3 View

What can be the reason of not getting any ban blotnd after developing a wester

Hi all I have been facing a lot of problems with my western blot development. 1) First I failed to develop my target protein on x-ray although every time I got a fine band for my control(beta...

01 January 1970 4,999 1 View