Reason why my Plasmids isolated using alkaline lysis didn't dissolve in water?

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Which one is a better journal metric CiteScore or Impact Factor?

CiteScore calculation is based on Scopus data, while Impact Factor is based on Web of Science data. For example, Journal of Intellectual Capital has a CiteScore of 8.6 and an Impact Factor of...

01 July 2020 5,536 6 View

What is the best cell confluence at which we should treat our running cancer cells with CoCl2 to mimic hypoxia mediated conditions?

Hello.... I am intending to mimic hypoxia induced conditions in cancer cells with CoCl2 (for 24 hrs) and then re-oxygenate the CoCl2 treated cells with fresh culture media without CoCl2. I am...

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30 March 2017 1,443 5 View

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31 October 2016 4,529 3 View

Hello, can anyone assist me about the exact conditions to develop a western blot, using Luminata forte?

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Is there any assay to quantify the NADPH produced by the neutrophils?

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Need of Agrobacterium AGL-1 strain with pBHt2 vector for Colletotrichum lindemuthianum transformation?

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26 January 2016 8,015 2 View

Is there a protocol to assess the effect of sodium chloride on the activity of salivary amylase?

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22 September 2014 8,466 1 View

Can anyone suggest how to do standardization of EST SSR's?

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04 November 2013 3,114 1 View

What can be the reason of not getting any ban blotnd after developing a wester

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What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

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Chlorinated organic formation in odor control scrubbers using hypochlorite?

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Does Napabucasin soluble in dichloromethane and in ethanol?

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Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

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01 March 2021 210 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

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01 March 2021 9,310 4 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

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Expression cloning by using cDNA,do I need to modify the cDNA first?

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Is there a method of determining feed digestibility without the need to re-pellet the feed?

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Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

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PUC19 as a transfer plasmid for lentiviral delivery?

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Michael J. Benedik

It sounds like your plasmid did dissolve just fine in water, otherwise you would not have such a high concentration reading with the nano drop. The precipitate is probably some salt or other molecule that is less soluble. You could try and and just pellet the ppt to remove it and double check the solution again.

Alireza Mordadi

I suggest you increase your final vol maybe your con of DNA is more than this.