My hela line grows very slowly.
I changed my source and got new source of hela line from another institute, but when I passage this new source, the new flasks grow slowly again.
what can I do for this problem?
My HeLa cells grow at 10% FBS in DMEM.
Why don't you try using dishes instead of flask?
Hela cells have been passaged multiple times in multiple places and some stocks have been in culture for a very long time without interruption. Over time the different Helas being used have different phenotypes, morphology and growth characteristics. This problem is specific to Helas and in some places, differences in hela strains are considered normal
I have agree with previous answer to must be checked nutrient component in medium especially FBS (concentration and brand). and also checked incubation condition.
You need to check the quality and contamination of micoplasma of the medium and serum. If you use an antibiotic, then adjust the dosage. Maybe you make a lot of antibiotics. Check out the growing conditions of the cells (temperature) and quality cookware. Pay attention to the volume of the medium over the cells (probably insufficient gas exchange in the environment).
What is the concentration of glucose in the medium that you use? Do you add some powders into the medium? I had some problems also with glutamine or power-glucose added. So I think that also these two factors are important. However, I have always used 10% FBS, but more passages can compromise phenotype, cell growth and response to your treatments.
I would also recommend obtaining a fresh batch of HeLa cells from ATCC, if possible. Whenever you get a cell line from a secondary or tertiary source, you run the risk of getting an impure cell line, or a cell line that has been maintained (even inadvertently) to select for high/low density, fast/slow growth.
Hela may be contaminated with mycoplasma so growing slowly. Do check other cell lines in incubator if they are also contaminated with mycoplasma. It may be source of your problem. Also use 15% Fetal bovine serum instead of 10% to boost the growth.
You may try fresh hela cells insted of getting them from secondary or other sources or may check the composition of nutrients in the medium.
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