Which contaminative agents must be test before use cell lines for tumor engraft in nude mice?

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Can shRNA-plasmids contain multiple domains of a single gene with one hairpin and under the control of a single promoter?

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Why can I not confirm my positive colonies (shRNA ligated in pRNAi vector) via PCR but using a RE can confirm the same positive colonies & plasmids?

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Can anyone advise on how to clone a small 80 bp segment in a big 4800 bp plasmid?

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Why I can not find my insert (80 bp shRNA) in plasmid (5000 bp pRNAi) via colony pcr?

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How long does it take for tetracyclin to induce the RNAi vector and knock down the gene?

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Hela cell line growing slowly

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31 December 2012 967 10 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

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IHC on mouse liver hepatocytes?

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How to avoid ulcers in s.c. injected tumors ?

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The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

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What is the exact mechanism of the antimicrobial effect of local anesthetics?

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Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

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What are the pros and cons for mice versus rats?

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Is it harmful to breed for Cre Homozygosity initially?

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Martin Klvana

(Poor mice.)

Mohammad Mehdi Akbarin

At first for every cell line you should checked for Mycoplasma /Chlamydiae contamination, furthermore it would be used full if you could checked CMV among cell line. you can check them for your nude mice which are most talented for opportunistic infections.

Best regards

Steingrimur Stefansson

Hi Saeideh, 1) check for endotoxin, 2) check for endotoxin, 3) re-check for endotoxin.

Saeideh Milani

Thanks all for their guidance.