Amit Kulkarni

2 Questions 22 Answers 0 Followers

Questions related from Amit Kulkarni

Can anyone recommend using a lentivirus based vector for cloning 5 kb orf and it's expression?

I am looking for lentivirus based system to clone 5 kb orf gene (membrane protein). From my reading, it appears to me that a lentivirus system with 4kb insert and above is unstable and...

10 October 2014 5,598 2 View

What is best transfection reagent for co-transfection or sucessive transfection of siRNA and DNA in Hela cells ?

I was wondering if someone has optimised a protocol for co-transfection of DNA and siRNA in Hela cells ? I am attempting a rescue experiment were siRNA treated Hela cells are transfected with the...

02 February 2014 8,864 9 View