7 Questions 9 Answers 0 Followers
Questions related from Saeideh Milani
Dear all I want to induce lymphoma in nude mice as an animal model.Can any one guide me before inoculation of related cell lines into nude mice which contamination biological agent such as...
07 July 2017 1,734 4 View
I want to construct an shRNA-plasmid containing multi-domains of a single gene with one hairpin and under the control of single promoter. Is it possible?
03 March 2014 7,806 1 View
For confirmation the cloning of my insert (shRNA) in pRNAi vector, PCR did not respond at all, but when I tested the extracted plasmids of the same colonies with restricted enzyme (scaI), I found...
10 October 2013 1,407 3 View
When I digest the ends of the segment & the plasmid with restriction enzymes, they can not ligate together and although some times I have clones when I use colony PCR I do not have any band.
09 September 2013 2,454 7 View
I do not detect my insert in my colonies via colony pcr. What is the reason? I wanted to clone a shRNA in pRNAi. I digested my plasmid & pcr product of shRNA with the same RE. Then ligate them...
09 September 2013 5,246 1 View
I transfected a cell line with pRNAi vector and then added tetracyclin as an inducer of its promoter. How long do I have to wait if I want tetracyclin to induce the promoter of my plasmid and...
02 February 2013 5,676 3 View
My hela line grows very slowly. I changed my source and got new source of hela line from another institute, but when I passage this new source, the new flasks grow slowly again. what can I do...
01 January 2013 1,005 10 View
