Hello, I am making organotypic slices, and for some import-related issues, there is no available heat-inactivated horse serum that I can receive soon. For that, I thought about heat inactivating a horse serum on my own in the lab. I need to get inputs and advice for that. the protocol I find is heating at 56c for 30 min. Is there a way/test to check if I did that correctly before using it in my media? also, i am wondering if I have to do that freshly? or can I do that in batches and aliquot them in the freezer for example?

thanks!