2 Questions 8 Answers 0 Followers
Questions related from Christiane Schnabel
I have a simple western blot of CD31 in HUVEC lysate. With an anti-mouse-HRP secondary Ab I get a good signal/band. With fluorescent secondary antibody there is no signal. I have tried multiple...
06 August 2019 6,187 3 View
From transient e get very high levels of secreted recombinant protein with a N-terminal his-tag, but our purification results are poor (less than 5% yield). We have tried desalting the...
30 January 2017 9,732 12 View
