I am analyzing the levels of secreted HBsAg in the hepg2.215 culture supernatant by commercial enzyme immunoassays (Monolisa™ HBs Ag ULTRA). My questions are:

1) Can i perform the test in a 96 well plate?

2) What is the incubation period required before taking the supernatant?

3) What is the minimum number of cells that can be used (seeded) in the well plate?

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