I am analyzing the levels of secreted HBsAg in the hepg2.215 culture supernatant by commercial enzyme immunoassays (Monolisa™ HBs Ag ULTRA). My questions are:
1) Can i perform the test in a 96 well plate?
2) What is the incubation period required before taking the supernatant?
3) What is the minimum number of cells that can be used (seeded) in the well plate?