Ahmed H. Arbab

15 Questions 18 Answers 0 Followers

Questions related from Ahmed H. Arbab

Can you answer my questions on a protocol for screening of anti-HBV activity by analyzing of HBsAg and HBeAg expression?

The protocol is for time dependent study for screening of anti-HBV activity by analyzing of HBsAg and HBeAg expression (HepG2.2.15, 96 well plate)? 1- Time course (duration of study) to measure...

13 January 2015 6,572 1 View

Can I use DMSO as solvent to prepare stock solution of plant extract for antioxidant screening ?

Can I DMSO as solvent to prepare stock solution of different plant extract for antioxidant screening??

14 November 2014 5,336 11 View

What is the the role of antioxidant activity in the mechanism of antiviral (eg. Anti-HBV) natural product ?

I am asking about the role of antioxidant activity in the mechanism of antiviral (eg. Anti-HBV) natural product. Can Antioxidant potentiate/ contribute to antiviral activity?

27 October 2014 5,462 4 View

What is the role of hepatoprotective product in treatment/management of hepatitis B virus infection?

What is the role of hepatoprotective products in treatment/management of hepatitis B virus infection?

18 August 2014 6,207 7 View

Why iam getting sudden cell death in an MTT assay after incubation?

In MTT cell proliferation assay, after seeding cells (eg.HepG2) in 96 well plates (24h) and treating cells with drugs under study and incubation for 48hrs, sudden cell death occurs, including...

27 May 2014 8,418 5 View

Can I use a wavelength of 490 nm for DPPH antioxidant reading?

We know that the optimum wavelength (lambda max) for DPPH reading is 517 nm. Can I use 490 nm (517 liter is not available in my lab)?

13 May 2014 9,545 8 View

Can anyone help with an antioxidant protocol?

I am asking about a protocol for antioxidant activity assay (DPPH, CUPRAC, and Bocarotene/linoleic acid assay) using 96well plate.

08 May 2014 8,106 4 View

HBsAg assay/hepg2.215 cell line?

I am analyzing the levels of secreted HBsAg in the hepg2.215 culture supernatant by commercial enzyme immunoassays (Monolisa™ HBs Ag ULTRA). My questions are: 1) Can i perform the test in a 96...

07 November 2013 2,279 3 View

Bioautography - can anyone help?

I am asking about the recent applications of bioautography (Bioautography on thin-layer chromatographic (TLC) plates as a mean of detecting the biological activity), Is there any application...

18 September 2013 9,304 1 View

How to measure cell culture based in vitro Hepatoprotective actvity?

How can I measure in vitro hepatoprotective activity of a phytoprodct (extract, pure compounds) in a lvier cell line (HepG2)/ what are the suitable agents that can be used to induce cell injury...

14 September 2013 6,831 2 View

Suitable oncertrations of plant extract (80% ethanolic extract) for cytotoxicity (MTT method).

What concentrations of plant extract (80% ethanolic extract) should be used in 96 well plates for a cytotoxicity study (MTT method, Hepg2 cell line)? What are the suitable controls?

15 February 2013 9,675 5 View

In vitro / cell culture models for HBV

I am asking about suitable in vitro/ cell culture models f for studying anti-HBV activities of certain compounds

09 October 2012 7,233 4 View

Do you know where I can get the HepG2.2.2.15 cell line?

Do you know where I can get the HepG2.2.2.15 cell line? HepG2 cells transfected with HBV DNA.

03 September 2012 7,667 1 View

Liquid liquid fractionation for plant extract

Hi all friends i am asking for a procedure for liquid- liquid fractionation for a polar(methanol extract) thermolabile plant extract , i want to fractionate the extract to 5-6 fractions to ease...

13 January 2012 8,888 7 View

Which is the best method for in screening for anticancer activity of plant extract

I want to test a pant extract for anticancer activity in cell culture

02 January 2012 9,419 13 View