12 Questions 15 Answers 0 Followers
Questions related from Ahmed H. Arbab
The protocol is for time dependent study for screening of anti-HBV activity by analyzing of HBsAg and HBeAg expression (HepG2.2.15, 96 well plate)? 1- Time course (duration of study) to measure...
13 January 2015 6,511 1 View
Can I DMSO as solvent to prepare stock solution of different plant extract for antioxidant screening??
14 November 2014 5,279 11 View
What is the role of hepatoprotective products in treatment/management of hepatitis B virus infection?
18 August 2014 6,164 7 View
In MTT cell proliferation assay, after seeding cells (eg.HepG2) in 96 well plates (24h) and treating cells with drugs under study and incubation for 48hrs, sudden cell death occurs, including...
27 May 2014 8,374 5 View
I am asking about a protocol for antioxidant activity assay (DPPH, CUPRAC, and Bocarotene/linoleic acid assay) using 96well plate.
08 May 2014 8,068 4 View
I am analyzing the levels of secreted HBsAg in the hepg2.215 culture supernatant by commercial enzyme immunoassays (Monolisa™ HBs Ag ULTRA). My questions are: 1) Can i perform the test in a 96...
07 November 2013 2,237 3 View
I am asking about the recent applications of bioautography (Bioautography on thin-layer chromatographic (TLC) plates as a mean of detecting the biological activity), Is there any application...
18 September 2013 9,268 1 View
How can I measure in vitro hepatoprotective activity of a phytoprodct (extract, pure compounds) in a lvier cell line (HepG2)/ what are the suitable agents that can be used to induce cell injury...
14 September 2013 6,785 2 View
What concentrations of plant extract (80% ethanolic extract) should be used in 96 well plates for a cytotoxicity study (MTT method, Hepg2 cell line)? What are the suitable controls?
15 February 2013 9,632 5 View
I am asking about suitable in vitro/ cell culture models f for studying anti-HBV activities of certain compounds
09 October 2012 7,188 4 View
Hi all friends i am asking for a procedure for liquid- liquid fractionation for a polar(methanol extract) thermolabile plant extract , i want to fractionate the extract to 5-6 fractions to ease...
13 January 2012 8,850 7 View
I want to test a pant extract for anticancer activity in cell culture
02 January 2012 9,350 13 View