Have you got the sequence of control PCR fragment that comes up with InsTAclone PCR Cloning Kit - Thermo Fisher Scientific?

More Sarbesh Das Dangol's questions See All

How online classes have been doing after the pandemic? What kind of variables can we study related to it?

Developing countries adopted online learning just because of pandemic and most probably those nations opt-out from online learning and teaching after the situation settled down. So, what kind of...

13 March 2023 4,459 2 View

Can USDA still miss transgene detection while regulating final products of GMO and gene edited crops?

USDA checks for promoters and transgenes in the final product (after selfing, backcrossing, etc.) before releasing it to the market. Do they check for every backbone in the vector? Do they check...

21 June 2021 259 1 View

How to solve contact problem in ANSYS APDL?

I am trying to simulate three edge bearing test of a concrete pipe in ANSYS APDL. I have the pipe resting on two bearers (defined as contact element) and displacement load applied directly on top...

24 September 2020 6,029 2 View

What are the differences between rules and regulations?

What are the differences and similarities between rules and regulations? Also, I need good references (articles/textbook) that illustrate the differences and similarities. Thanks.

08 March 2019 6,814 2 View

Can a high mutation rate in a GOI for a particular variety of a crop induced by CRISPR knockout be related with high mutation rate for that variety?

Can a high mutation rate in a GOI for a particular variety of a crop induced by CRISPR knockout be related with high mutation rate for that variety with this technology? Are there any papers that...

20 September 2017 304 0 View

Can M6 diploid potato line be transformed using Agrobacterium?

M6 (Reg. No. GP-1, BS 228) is a diploid self-compatibleinbred line of the potato wild relative Solanum chacoense. Could you please let me know if you have successfully transformed this line using...

05 July 2017 8,660 2 View

How efficient is information generated from 25% of whole genome? Is it necessary to conduct whole genome sequencing & BAC clone generation vis-à-vis?

"Specifically, we interrogated a large set of whole-genome shotgun (WGS) sequence reads representing approximately 25% of the sunflower genome, as well as the sequences of 21 unique bacterial...

14 May 2016 8,312 1 View

What are the differences between these terms?

What is the difference between water potential (MPa) and relative water content (%) in plants? Also, how stomatal conductance (mmol/m2s) and canopy temperature (o C) are co-related?

19 April 2016 3,541 3 View

Do tetraploid/ tetrasomic potatoes suffer from inbreeding depression as frequently as do the diploids during the conventional plant breeding?

Do tetraploid potatoes suffer from inbreeding depression as frequently as do the diploid potatoes during the conventional plant breeding? Please explain. Also, can you please elaborate on...

28 January 2016 4,373 1 View

Are right TALEN and left TALEN harbored in separate expression vector plasmids and delivered together in protoplast for mutational studies?

Are right TALEN and left TALEN harbored in separate expression vector plasmids and delivered together in protoplast for mutational studies? Or are both of these TALEN pairs designed to be...

10 January 2016 9,808 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Is it true that the vacuum bomb is about to arrive?

We assume this to be true. We also assume that the vacuum bomb is the latest version of explosives with an explosive power of a few to ten kg of TNT equivalent. It has the unique characteristic of...

04 August 2024 4,534 1 View

How can we now if the promotor in the vector is well?

Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...

02 August 2024 3,987 1 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

Dimensions of an MJ Research 96-well alpha module?

Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...

30 July 2024 2,867 0 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 841 2 View

Someone have the key or the installer of the program Mx pro 3000 (PCR real time)?

I need to install this program

25 July 2024 4,756 0 View