23 Questions 8 Answers 0 Followers
Questions related from Sarbesh Das Dangol
USDA checks for promoters and transgenes in the final product (after selfing, backcrossing, etc.) before releasing it to the market. Do they check for every backbone in the vector? Do they check...
22 June 2021 289 1 View
I have a gene of interest that has higher AT-rich composition. Also, GOI has 1-4 mismatches for the "seed sequence". I think this is the factor that the sgRNA scoring is relatively low, 0.5..,...
23 January 2018 1,930 0 View
Can a high mutation rate in a GOI for a particular variety of a crop induced by CRISPR knockout be related with high mutation rate for that variety with this technology? Are there any papers that...
21 September 2017 326 0 View
M6 (Reg. No. GP-1, BS 228) is a diploid self-compatibleinbred line of the potato wild relative Solanum chacoense. Could you please let me know if you have successfully transformed this line using...
06 July 2017 8,686 2 View
Is it required that we target all four alleles of a gene in tetraploid potato desiree for knockout studies using CRISPR/Cas9 technology? How effective is CRISPR/Cas9 vector transformation in...
12 April 2017 5,102 3 View
Can you please suggest me some commercial CRISPR/Cas9 vectors suitable for potato transformation via Agrobacterium?
24 May 2016 7,138 3 View
Can it be assumed that the sequence information derived from randomly chosen Bacterial Artificial Chromosome (BAC) clones are representative of the genome as a whole? If so, how much error is...
16 May 2016 2,642 3 View
"Specifically, we interrogated a large set of whole-genome shotgun (WGS) sequence reads representing approximately 25% of the sunflower genome, as well as the sequences of 21 unique bacterial...
15 May 2016 8,350 1 View
How efficient is Agrobacterium in expressing foreign proteins from non-plant members such as algae and cyanobacteria in potato chloroplast? Can you please suggest me some reliable protocols if any?
29 April 2016 1,884 6 View
What is the difference between water potential (MPa) and relative water content (%) in plants? Also, how stomatal conductance (mmol/m2s) and canopy temperature (o C) are co-related?
20 April 2016 3,568 3 View
I've read from several papers that sunflower plants are cumbersome to work with when it comes to Agrobacterium mediated genetic transformation, albeit there are many protocols developed, but not...
04 March 2016 5,370 0 View
Do tetraploid potatoes suffer from inbreeding depression as frequently as do the diploid potatoes during the conventional plant breeding? Please explain. Also, can you please elaborate on...
29 January 2016 4,397 1 View
Are right TALEN and left TALEN harbored in separate expression vector plasmids and delivered together in protoplast for mutational studies? Or are both of these TALEN pairs designed to be...
11 January 2016 9,829 1 View
To what extent does methylation affect TALEN pairs and CRISPR/Cas9 systems? Are there reports of significant off-target mutagenesis? Kindly elaborate on the pros and cons of these gene editing...
09 January 2016 8,984 2 View
Can you please tell me with reasons on how challenging it is to sequence heterozygous tetraploid potatoes with respect to its allelic diversity? Does 454 deep sequencing effectively help in...
04 January 2016 7,858 1 View
An SLR1 promoter is a tissue-specific promoter for exocyst genes which is strongly expressed only in stigma and not in the pollen or style. Suppose that we have an RNA knockout gene construct as...
03 January 2016 1,318 0 View
Sugars regulate gene transcription by modulating promoter activities. Conserved cis-elements have been identified in the promoters of many sugar-regulated genes -- SURE motif (sugar-responsive...
28 December 2015 368 3 View
Silencing CWIN expression in tomato resulted in increased fruit abortion, and reduced fruit size and seed number per plant (Zanor et al., 2009). An article from a team led by Dr. Yong Ling Ruan in...
28 December 2015 5,946 1 View
Can you please briefly and concisely describe why triploids and pentaploids are genetically sterile and what are the molecular mechanisms behind this?
14 December 2015 5,457 3 View
Kindly let me know if GV3101-pMP90RK Agrobacterium strain works for transformation of Benthamiana.
12 December 2015 3,555 3 View
I have encountered many articles that have conducted inductive expression of transcription factors by cloning their respective inductive promoters -- constitutive expression is detrimental to...
12 December 2015 5,191 1 View
I need sequence of control PCR fragment that comes up with InsTAclone PCR Cloning Kit - Thermo Fisher Scientific (plasmid vector pTZ57R/T). Does anyone have this? (#K1213)
14 October 2015 1,744 3 View
Many papers and standard lab protocols describe growing Agrobacterium at 28 degrees. However, few papers also describe doing the same at 29 degrees, and some protocols give a freedom of 28-30...
01 January 1970 509 2 View
