I have occasionally experienced that the two cell lines have grown in very regular wavy lines from the stopper to the opposite end of the flask. The cells seems to be okay except that they won't grow on the majority of the area in the T75 flask.
Do you think it is the coating of the flask or the cells that is the problem?
The flask is a standard culture flask from Corning Inc.
Should I just split the cells into a new flask and otherwise ignore it?
/Kristian Mark Jacobsen