Lately, I have gotten a problem with transfection using Lipofectamine 2000. The transfection efficiency is obviously dropped comparing to my past experiments. I have tried varying the condition but it was never as good as before. However, I have noticed something strange in my experiments that there are something on my transfected cells which I assumed that they might be lipofectamine-DNA complex. (none was shown on untransfected cells.)
I don't know if it was my mistake this was happened, or this was normal.
I've never thought that lipofectamine-DNA complex is visible?
Is this the reason behind my low transfection efficiency?
Yes! I have definitely seen these complexes. They are able to be seen if one looks for small dark dots floating in the media. It might require changing the focus, yet they are there. They can also be seen with other transfection reagents. However, I'm not sure I've ever seen them on the surface of cells. Moreover, I'm unsure whether they have anything to do with your transfection efficiency, as I've seen them in both very efficient and not-so-efficient transfections.
Yes! I have definitely seen these complexes. They are able to be seen if one looks for small dark dots floating in the media. It might require changing the focus, yet they are there. They can also be seen with other transfection reagents. However, I'm not sure I've ever seen them on the surface of cells. Moreover, I'm unsure whether they have anything to do with your transfection efficiency, as I've seen them in both very efficient and not-so-efficient transfections.
- Badges
- Science method