4 Questions 11 Answers 0 Followers
Questions related from Wareerat Umnajvijit
Lately, I have gotten a problem with transfection using Lipofectamine 2000. The transfection efficiency is obviously dropped comparing to my past experiments. I have tried varying the condition...
12 December 2016 9,869 2 View
My previous result found that in the stable cell line expressing my protein, basal protein level of the target protein is decreased and the decreasing is more prominent in those cell expressing my...
10 October 2016 9,034 1 View
I have thawed 2 vials of cell which were kept at -80C. After the spin cell down, to remove frozen medium, I refilled with a new completed medium and I found some suspicious things which are bigger...
07 July 2013 599 19 View
I have a problem with plasmid extraction by column. I am trying to extract plasmid by phenol-chloroform. I got the following protocol from nearby lab: 1. Resuspended bacterial plasmid with...
02 February 2013 7,159 9 View