Is there any free geographic information system (GIS) software to map epidemiology of a disease and its features on a country map?

08 September 2016 8,433 7 View

What is best way to correlate real time PCR values with biochemical features?

Hello everyone I would like to ask question about situations where gene expression values are used to correlate with clinical features of samples under investigation. For example, in an...

31 December 2014 3,972 5 View

Why am I getting aqueous phase contamination with Trizol?

I am using the QIAGEN miRNeasy kit for total RNA isolation from tissue and serum samples as per manual instructions. However, I am facing issues with the phenol contamination and the 260/230...

04 May 2014 206 7 View

Can anyone advise me on codon usage analysis, ENC vs GC3 values?

In codon usage studies, it is observed that a correlation between ENC and GC3 values is plotted as a curve following Wright's, 1990 method and hypothesis. However, what should be the case when a...

11 December 2013 699 1 View

Rights of students enrolled in Universities and Research Institutes?

I would like to have your opinions on rights of students when they are enrolled in different degree programs considering following case as an example: 1. A student is studying for his/her higher...

08 September 2013 9,197 9 View

Why is the polynomial trend line inverted?

I am trying to perform the correspondence analysis between the set of values of GC3 and ENC, given in the attached Microsoft Excel sheet. The problem is that I have applied trend line (polynomial)...

07 August 2013 6,048 2 View

Could anyone please tell me if is there any tool/server available for miRNA target prediction in user specified genomes?

There are several target prediction tools but they are limited in terms of options that we can select humans, mouse etc genomes. What if the genome sequence of any other organism, which is not...

06 July 2013 9,876 2 View

COX protein isolation from Blood/serum

Hello everyone Could anyone please tell me is it possible to isolate COX protein from serum or whole blood samples for western blotting?

02 March 2013 8,362 2 View

Annotation of viral genomes

Please suggest a reliable free software/server for annotation of newly sequenced viral genomes

01 February 2013 2,232 7 View

GFF annotation files

I would like to know how to download GFF or GTF files of annotated full length viral genomes from NCBI?

01 February 2013 3,168 4 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View