Is there any free geographic information system (GIS) software to map epidemiology of a disease and its features on a country map?

08 September 2016 8,433 7 View

What is best way to correlate real time PCR values with biochemical features?

Hello everyone I would like to ask question about situations where gene expression values are used to correlate with clinical features of samples under investigation. For example, in an...

31 December 2014 3,972 5 View

Has anyone used the spike-in for circulating microRNA?

I would like to know if there is anyone who has worked specifically with QIAGEN cel-miR 39 spike-in using the QIAGEN miRneasy mini kit.

04 May 2014 9,391 3 View

Why am I getting aqueous phase contamination with Trizol?

I am using the QIAGEN miRNeasy kit for total RNA isolation from tissue and serum samples as per manual instructions. However, I am facing issues with the phenol contamination and the 260/230...

04 May 2014 206 7 View

Can anyone advise me on codon usage analysis, ENC vs GC3 values?

In codon usage studies, it is observed that a correlation between ENC and GC3 values is plotted as a curve following Wright's, 1990 method and hypothesis. However, what should be the case when a...

11 December 2013 699 1 View

Rights of students enrolled in Universities and Research Institutes?

I would like to have your opinions on rights of students when they are enrolled in different degree programs considering following case as an example: 1. A student is studying for his/her higher...

08 September 2013 9,197 9 View

Why is the polynomial trend line inverted?

I am trying to perform the correspondence analysis between the set of values of GC3 and ENC, given in the attached Microsoft Excel sheet. The problem is that I have applied trend line (polynomial)...

07 August 2013 6,048 2 View

Could anyone please tell me if is there any tool/server available for miRNA target prediction in user specified genomes?

There are several target prediction tools but they are limited in terms of options that we can select humans, mouse etc genomes. What if the genome sequence of any other organism, which is not...

06 July 2013 9,876 2 View

Annotation of viral genomes

Please suggest a reliable free software/server for annotation of newly sequenced viral genomes

01 February 2013 2,232 7 View

GFF annotation files

I would like to know how to download GFF or GTF files of annotated full length viral genomes from NCBI?

01 February 2013 3,168 4 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View