How did people get on with these? Any issues with the 4ug column retention?
I've plotted my data and it looks to be good for a random sequential mechanism. When plotted in excel it fits this trend nicely. However, when I plot these in SigmaPlot, it does not want to put...
05 June 2017 561 3 View
Hello, So I recently expressed a protein of about 25 kDa. I first purified it through Ni-NTA affinity column, then ran O/N dialysis, then for a final stage of purification (because I want to...
04 May 2017 10,073 8 View
Has anyone ever used ZipTips to concentrate or purify a protein? I can't think of any reason why this wouldn't work if you use the correct tip and solvent. As a benchmark, my protein is 23 kDA....
02 March 2017 518 1 View
So I work in an enzymology lab in a chemistry dept. We do not have much experience with cladistic analysis, however it was suggested by a reviewer of a paper that it would help some readers,...
31 December 2016 4,673 5 View
I am about to perform a protein expression experiment and right now what I have is my gene in a pUC57 vector. As I'm not experienced in the procedure, I wanted to ensure I had backup DNA to work...
01 February 2016 4,449 5 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View