Brian O' Flynn

6 Questions 3 Answers 0 Followers

Questions related from Brian O' Flynn

Can anyone help me with an enzyme kinetics sigmaplot fit?

I've plotted my data and it looks to be good for a random sequential mechanism. When plotted in excel it fits this trend nicely. However, when I plot these in SigmaPlot, it does not want to put...

06 June 2017 547 3 View

One protein but three peaks on FPLC chromatogram?

Hello, So I recently expressed a protein of about 25 kDa. I first purified it through Ni-NTA affinity column, then ran O/N dialysis, then for a final stage of purification (because I want to...

05 May 2017 10,052 8 View

Protein purification/concentration using ZipTips?

Has anyone ever used ZipTips to concentrate or purify a protein? I can't think of any reason why this wouldn't work if you use the correct tip and solvent. As a benchmark, my protein is 23 kDA....

03 March 2017 500 1 View

When forming a phylogenic tree (cladogram) using MEGA, what kind of model would best suit?

So I work in an enzymology lab in a chemistry dept. We do not have much experience with cladistic analysis, however it was suggested by a reviewer of a paper that it would help some readers,...

01 January 2017 4,643 5 View

Has anyone used the new NEB Monarch DNA Gel Extraction Kits (T1020)?

How did people get on with these? Any issues with the 4ug column retention?

09 September 2016 5,366 0 View

Is there any way to store linear DNA product that I get from vector digestion as well as the PCR product so as I can keep them as a backup?

I am about to perform a protein expression experiment and right now what I have is my gene in a pUC57 vector. As I'm not experienced in the procedure, I wanted to ensure I had backup DNA to work...

02 February 2016 4,433 5 View