I started using this system for breast cancer cell migration. I treated the membrane with 30ug/ml rat tail collagen overnight and used 8000 cell per well. After 5 hours migration, I cleaned the nonmigrated cell by wiping the nonmigration side of the membrane 9 times by the rubber wiper with dipping in PBS between wiping. I got a lot of variations in the result. Sometimes no cell attached at all. I wonder if cleaning too much on nonmigration side will somehow affect the other side of the membrane. And collagen treatment time and dose make difference in this assay?
Hello! Which pore size are you using? I'll try 8 um, without collagen first. And I think that cleaning just about 3 times is OK. I am using it for melanoma cells, but it was previously used with breast cancer cells in my lab. Luck
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