I found this work and I really liked the results presented:
Article Rapid and zero-cost DNA extraction from soft-bodied insects ...
However, I didn't understand what should I do after incubating the sample at 98°C (2 min). I tried it with human stomach samples. I crushed 0,140 g of stomach mucosa in 200 uL of PBS (I think the buffer needs to cover all the sample, right?). After the incubation, I am not sure about what should I do because this is not clear to me on the work methodology. "All the lysates were incubated at 98˚C for 2 min in a water bath and assessed for purity, quantity, and PCR applications." I can not use the DNA because there is a lot of protein yet, as the rapid method didn't include any protein cleaning at the description.
They are using a membrane to bind the DNA and then eluting from it with the second 98 degree incubation step. I would suggest you use proteinase K during your initial lysis step to degrade most of the protein.
I think they used a membrane just as one of the five methods they tried... At the abstract they said that they compared five rapid DNA extraction methods with standard CTAB extraction buffer and DNeasy. One of the methods they cited is the Nitrocellulose Membrane (NCM). However, they do not mentioned any membrane concerning to the rapid method with the other solutions... I mean, the title says "a zero-cost DNA extraction". Table 2 showns that they extracted just with sterile water (SDW) with the cost of 0.0 US$. The membrane method is cited as a 0.012 US$ method haha
Apologies, the methods section of that paper is not clearly written. Seems they are just using the method used for colony PCR from E. coli on insect hemolymph. After the lysis by boiling you centrifuge your samples (usually 10 mins) to pellet most of the cellular debris and then use 5ul for your PCR. The method does not remove all proteins. If you want a low cost method to remove most of the remaining protein do an isopropanol precipitation, discard the supernatant, wash with 70% ethanol and then resuspend in fresh water or TE buffer.
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