Louise Magalhães Albuquerque

6 Questions 4 Answers 0 Followers

Questions related from Louise Magalhães Albuquerque

I'm having contamination issues when performing real-time PCR. How is this occurring?

I am doing microsatellite instability HRM on a Rotor Gene Q. I always run a negative control containing just the reagents and Nuclease Free water. The reagents are prepared in a workstation, that...

08 March 2023 7,190 1 View

Has anyone tried to extract DNA with this 1 step method?

I found this work and I really liked the results presented: Rapid and zero-cost DNA extraction from soft-bodied insects ... However, I didn't understand what should I do after incubating the...

10 October 2022 8,417 3 View

How to know how many ng/uL my 100 mM primer should be?

Hello! I want to use an old 100 mM concentrated primer for a PCR. There is probably some degradation due to time (6 years old). Thus I have quantified it in nanodrop so I could do a reliable...

09 September 2021 2,720 3 View

Why my HRM for MSI have a lot of peaks?

Hello there. So I performed HRM for MSI of DNA samples from Gastric Tumours with the following protocol: 95 °C/5 min, 40 cycles of 95°C/10s, 58°C/30s, 72°C/10s, and 1 cycle of 72°C/1min, followed...

25 July 2019 6,022 0 View

Is Rotor-gene a good platform for detection of MSI?

I am trying to detect MSI using Rotor-gene Q platform ( © QIAGEN) by HRM ( using BAT25 and BAT26 markers). But I haven't got good results. I have searched in literature and found no work that...

01 January 1970 2,822 3 View

Is there some Cost-Effective Microsatellite Instability Detection option?

I am writing to seek advice and guidance regarding an issue my laboratory is currently facing. Unfortunately, due to budget constraints, we are unable to pursue expensive methods for the detection...

01 January 1970 3,038 0 View