I have successfully manged to label my exosomes with the said dye however i am trying to figure out how to assess their uptake by recipient cells
I am trying to use Lipofectamine 2000 to transfect miRNA inhibitor (antagomir) in TK6 cell line hence I would like to know if there is anyone who has successfully transfected TK6 cells with this...
08 September 2019 1,264 1 View
I have extracted microrna from my samples using a kit from Qiagen however several literature have shown that RNA quality needs to be checked before proceeding to cDNA synthesis. Do I need to do...
04 May 2018 1,953 0 View
I have found the clinically relevant doses of anti cancer drugs I am interested however they are in mg/m2, I need to convert to M so I can use them in cell culture
02 March 2018 6,341 3 View
I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...
05 August 2024 3,290 1 View
What should be the zeta potential of bovine milk exosomes
25 July 2024 7,376 5 View
Hello, I want to ask regarding exosome protein quantification method. Is it necessary to lyse exosomes before measuring their protein contents using BCA assay kit? Or can I just straight away...
23 July 2024 4,603 4 View
Recently I have submitted an article regarding MB dye degradation by ZnO nanoparticle. I received a suggestion to include the discussion on the effect of pH. I want to know what is the impact of...
19 July 2024 4,868 1 View
These fungal slides are isolated from cultured Bread mold on PDA media and smear is treated with Lacto phenol Blue dye ..
13 July 2024 9,701 1 View
The harmful Dye pollution from industrial processes such as textile generated how to remove with magnetic biochar ?
07 July 2024 8,118 1 View
500 ng RNA is converted to cDNA to be genetically expressed by qpcr sybergreen dye, 1 ul primer F, 1 ul primer R, 10 ul master mix, 1 ul cdna template, and instead of a complete total volume...
06 July 2024 5,478 4 View
Hi, All, I am relatively new for exosome isolation. I used an izon column 70nm to isolate exosomes from differentiated neural cells, but the concentrations were pretty low after NTA analysis (I...
01 July 2024 2,558 0 View
Hi everybody, i need to pick your brains: i work with an adhesive fish cell line which forms a konfluent monolayer. I want to test an adsorbant substance, therefore i want to saturate the cell...
30 June 2024 9,300 0 View
I seek to understand the theoretical basis for selecting the optimal temperature for the thermal regeneration process of exhausted adsorbents, such as activated carbon loaded with dye molecules....
28 June 2024 1,867 3 View