I have successfully manged to label my exosomes with the said dye however i am trying to figure out how to assess their uptake by recipient cells
I am trying to use Lipofectamine 2000 to transfect miRNA inhibitor (antagomir) in TK6 cell line hence I would like to know if there is anyone who has successfully transfected TK6 cells with this...
08 September 2019 1,191 1 View
I have extracted microrna from my samples using a kit from Qiagen however several literature have shown that RNA quality needs to be checked before proceeding to cDNA synthesis. Do I need to do...
04 May 2018 1,877 0 View
I have found the clinically relevant doses of anti cancer drugs I am interested however they are in mg/m2, I need to convert to M so I can use them in cell culture
02 March 2018 6,263 3 View
Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?
01 March 2021 9,447 3 View
Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...
26 February 2021 9,119 3 View
I am using IZON qEV2 columns to isolate exosomes from 100 ml of cancer cell conditioned medium. in NTA analysis I usually see concentration of 2x10^9 particles/ml. But when I proceed with this...
20 February 2021 6,879 3 View
Hello, I'm working on removal of methylene blue. 01. in the dark test very first reading is taken 15 minutes after adding adsorbent to the dye solution and that UV absorbance reading is very...
19 February 2021 472 5 View
Hi there, my lab recently opted to use LavaLAMP from Lucigen instead of the Warmstart kit from NEB. However, Lucigen has yet to create a colormetric kit for RT-LAMP, and adding phenol-Red (pH...
17 February 2021 5,399 1 View
Hi, In literature I noticed that who stains haemocytes with FDA for 30 min then stop the reaction in ice while this does not apply for 15 min incubation time (or maybe authors did not write this...
08 February 2021 3,366 3 View
Hello Everyone, We are inexperienced in culture of MSCs trying to grow a sample from BM of a mare. It is the second time that after preparation of new medium (DMEM-F12 with high glucose - 10% Hy...
07 February 2021 9,989 7 View
I was doing MTT assay, and in the last day before adding the MTT; the cells looked good (or so I think), but after aspiration of the drug and addition of MTT dye I noticed that most cells seemed...
07 February 2021 3,846 4 View
Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...
04 February 2021 4,482 4 View
Hi guys I'm out of ideas here please help! I've never had consistent problem like this doing westerns before and I can't figure it out. The condition used: - First 4 lanes have 50ug protein in...
04 February 2021 2,297 3 View