Arinzechukwu Ude

4 Questions 3 Answers 0 Followers

Questions related from Arinzechukwu Ude

Are TK6 cells easy to transfect?

I am trying to use Lipofectamine 2000 to transfect miRNA inhibitor (antagomir) in TK6 cell line hence I would like to know if there is anyone who has successfully transfected TK6 cells with this...

09 September 2019 1,242 1 View

Has anyone tried labelling exosomes with exoglow membrane dye? If yes, how did you assess the uptake of the labelled exosomes?

I have successfully manged to label my exosomes with the said dye however i am trying to figure out how to assess their uptake by recipient cells

12 December 2018 3,427 0 View

When analysing microRNAs by RT-PCR, do you need to check the quality of microRNA sample using agarose gel electrophoresis?

I have extracted microrna from my samples using a kit from Qiagen however several literature have shown that RNA quality needs to be checked before proceeding to cDNA synthesis. Do I need to do...

05 May 2018 1,928 0 View

Does anyone know about conversion of drug concentrations from mg/metre squared to molarity (in cell lines)?

I have found the clinically relevant doses of anti cancer drugs I am interested however they are in mg/m2, I need to convert to M so I can use them in cell culture

03 March 2018 6,318 3 View