I tried plating the same batch of cells on a 24-well plate and a cell-stack 2. Both surfaces are tissue culture treated and coated overnight with laminin. The cells plated very well on the 24-well plate, but were loosely adhered on the CS2 and are falling off as I move the CS. Any ideas on why this would be? I'm not sure if the sheer forces of media in the larger format could account for the poor adhesion. Thanks for any thoughts!
Hi Daisy
What is your cell type and both (24 well plate and cell stack) the culture dishes are the same brand? We have seen a huge difference in stem cell attachment between the brands despite all coated with laminin.
Certainly, the stack is extra vulnerable to shear force, due to its size. Perhaps there could also be some environmental vibration that gets caught on the cell stack better than on the 24-well plate. Sometimes putting your dishes on a cushion, or a stack of paper towels can do wonders.
It might be low-level contamination too. If you by chance have 1 mycoplasma cell in 20 ml, then you would dilute it to zero in the 24-well plate. But in the cell stack, you'd see them.
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