Has anyone produced Macrophages from human bone marrow monunuclear cells ?

More Mihai Nicolae Podaru's questions See All

How do you think new technologies will affect traditional arts?

My goal is to discover ways to use the new technological discoveries in the arts. The research is part of a study for a PhD thesis

01 April 2024 6,877 0 View

Are there any travel data published by groups doing experimental archaeology?

Can anyone guide me towards existing datasets of dugout and plank boats travel time in open water?

10 June 2022 8,591 0 View

Is it the linearity preserved at fields’ transition: classical - quantum electrodynamics fields?

If two classical electromagnetic fields are linearly correlated; can we say the same thing about the fields which describe the same classical fields at quantum scale?

17 March 2022 5,001 0 View

Are the Gaussian states of a discrete variable quantum system some new significant quantum states ?

To each Gaussian state of a continuous variable quantum system, we associate a discrete counterpart in each odd-dimensional Hilbert space and investigate the continuous-discrete similitudes. For...

14 November 2021 10,013 9 View

Can someone help me with image analyses from CT?

Hello, Can someone with experience in CT image analysis help me to understand the elements of the pictures. It is a plate thermoformed from waste like ABS and PMMA polymers and fiberglass. I need...

29 July 2021 1,887 6 View

How to perform mitochondria immunostaining?

Hi everyone, I'm having some issues with an immunofluorescence staining of the mitochondria in murine cancer cells. I've tried both a MitoTracker and a Tom20 antibody and the signal is always...

04 July 2021 4,513 7 View

Items loading on another factor when adapting a scale. What can be some reasons for that?

I am adapting a scale ( TIntS scale from Pretz, 2014 -23 items) into my native language. After I have done the following steps ( Gudmundsson, E. (2009). Guidelines for translating and adapting...

17 June 2021 953 3 View

Triple gene knock-out/knock-down feasibility?

Hi everyone, I'd like to ask you all for some advice regarding the feasibility of a certain experiment I'm trying to do. I need to generate a KO cell line in which the expression of a certain...

15 November 2020 2,860 1 View

It is possible to scan with an profilometer a round material with 10 mm thickness and 63,5 mm diameter?

The material is a composite with short unoriented fibers based on PMMA, ABS as a matrix, and fiberglass as reinforcement.

28 October 2020 3,061 2 View

Should we collapse instruments in large samples?

Collapsing instruments in a system GMM model is useful in treating the problem of instrument proliferation. However, the instructions given to xtabond2 of Stata suggest that "In large samples,...

11 January 2020 4,963 3 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

How long will macrophages remain in the M1 state after treatment with 100 ng/mL of LPS?

In my experiment, I treated RAW macrophages with 100 ng/mL of LPS. After 24 hours, I collected the supernatant and replaced it with fresh media. I then quantified the cytokine levels of TNF and...

01 August 2024 476 2 View

CHO-K1 suspension adaptation protocol?

I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....

31 July 2024 3,756 0 View

How to prevent hemolysis after performing cardiac puncture to draw blood from mice in serum?

We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?

29 July 2024 8,402 4 View

Human macrophages cell surface markers?

I have a question about human macrophages cell surface markers.I want to stain the unpolarized human macrophages for flow cytometric sorting without permeabilizing them. It is because I need to...

29 July 2024 236 2 View

I am working on my Master's thesis on the biogeography of the genus Ruagea and I would like to ask, could someone help me to check whether my result?

I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...

28 July 2024 2,979 1 View