Mihai Nicolae Podaru

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Questions related from Mihai Nicolae Podaru

Has anyone produced Macrophages from human bone marrow monunuclear cells ?

I am interested in the medium and serum choice as well as the concentration of M-CSF. Also, has anyone polarised these cells to an M2-like macrophage phenotype ? I used DMEM + 10% FBS and the...

03 April 2019 1,648 2 View

When do rat BM-MNC become able to attach to the culture flask in the process of M2 macrophage generation ?

Hello ! I'm trying to obtain M2 polarized macrophages from freshly isolated rat BM-MNC by initial treatment with M-CSF and then with IL-4. I believe freshly isolated BM-MNC are not able to attach...

29 February 2016 6,031 1 View