Hello all.
I have read a protocol stating that post paraformaldehyde fixation (4% in PBS) cell culture slides can be preserved in 70% ethanol at -20 deg. cel. until use (not good for long term, but has no effect on cells in the short term). The slides will be used for a TUNEL assay. My question is, has anyone had any experience with this kind of storing method, and if not, do you know if any other methods for storing slides post cell fixation, but prior to the actual staining.
Thank you
Ori Elad
Dear Elad,
I have used the same protocol for cell fixation and permeabilization for TUNAL assay and its works well. The only important step is to give a wash followed by paraformaldehyde treatment, otherwise the trace of PFA get crystalized and will hamper the process further.
vivek
- Badges
- Science topic