03 March 2015 7 5K Report

Hello all. 

I want to test whether two different factors - concentration (3 treatments) and incubation time (2 treatments) have an effect on gene expression. I ran the gene, and I have the Ct values for each treatment plus the Ct values of a house keeping gene for each treatment. both with 3 biological repeats and 3 technical repeats (a total of nine readings for each treatment for each gene). I have an efficiency factor for both, and I ran REST 2009 to see the difference in normalized expression. However, I want to run an ANOVA test to see the effect (if any) of each treatment and their interaction. What is the proper manipulation of data I should carry out?

Thank you. This is warping my brain for several weeks now. 

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